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Exposed Regions in Nonactivated and Phospho-Activated PhK a
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Exposed Regions in Nonactivated and Phospho-Activated PhK a
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Exposed Regions in Nonactivated and Phospho-Activated PhK a

Journal: Biochemistry

Article Title: Mass Spectrometric Analysis of Surface-Exposed Regions in the Hexadecameric Phosphorylase Kinase Complex

doi: 10.1021/acs.biochem.5b00682

Figure Lengend Snippet: Exposed Regions in Nonactivated and Phospho-Activated PhK a

Article Snippet: Enzymes and Reagents All proteases were from commercial sources: chymotrypsin (Ch), trypsin (Tr), and endoproteinase Arg C (RC) from Worthington Biochemical Corp. (Lakewood, NJ); ficin (Fi) and thermolysin (Th) from Sigma-Aldrich Corp. (St. Louis, MO); protease V8 (V8) from ICN Biomedicals (Irvine, CA); and endoproteinase Lys C (KC) from Promega Corp. (Madison, WI).

Techniques: Concentration Assay